Difference between revisions of "Part:BBa K2812006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Original DNA sequence based on ''Pseudomonas aeruginosa'' PA01 genome sequence, the nucleic acid sequences at positions 1067817–1066321 (the gene number: PA0985). This DNA sequence is codon optimized for E. coli. | + | Original DNA sequence based on ''Pseudomonas aeruginosa'' PA01 genome sequence, the nucleic acid sequences at positions 1067817–1066321 (the gene number: PA0985).<sup>1</sup> This DNA sequence is codon optimized for E. coli. |
===Source=== | ===Source=== | ||
Revision as of 00:55, 17 October 2018
Coding sequence for Pyocin S5 with HlyA and His6-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
Illegal BglII site found at 425
Illegal BglII site found at 2110 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1200
Design Notes
Original DNA sequence based on Pseudomonas aeruginosa PA01 genome sequence, the nucleic acid sequences at positions 1067817–1066321 (the gene number: PA0985).1 This DNA sequence is codon optimized for E. coli.
Source
Genomic sequence from Pseudomonas aeruginosa. DNA construct produced by IDT.
References
1) Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.