Difference between revisions of "Part:BBa K2812006:Design"
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===Source=== | ===Source=== | ||
| − | Genomic sequence from Pseudomonas aeruginosa. DNA produced by IDT. | + | Genomic sequence from ''Pseudomonas aeruginosa''. DNA construct produced by IDT. |
===References=== | ===References=== | ||
Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358. | Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358. | ||
Revision as of 00:51, 17 October 2018
Coding sequence for Pyocin S5 with HlyA and His6-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
Illegal BglII site found at 425
Illegal BglII site found at 2110 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1200
Design Notes
DNA sequence codon optimized for E. coli.
Source
Genomic sequence from Pseudomonas aeruginosa. DNA construct produced by IDT.
References
Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.