Difference between revisions of "Part:BBa J72003:Design"

(→‎Source)
(→‎Source)
 
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===Source===
 
===Source===
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<pre>
 
PCR ca1182F/ca1182R on pG80 CRIM plasmid  (BglII/XhoI)
 
PCR ca1182F/ca1182R on pG80 CRIM plasmid  (BglII/XhoI)
 
Sub into pBca9145-Bca1144#5              (BglII/XhoI,L)
 
Sub into pBca9145-Bca1144#5              (BglII/XhoI,L)
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ca1182R BBb biobricking of R6K
 
ca1182R BBb biobricking of R6K
 
gcaaaCTCGAGttaGGATCCgtgattcgcacgggcccatg
 
gcaaaCTCGAGttaGGATCCgtgattcgcacgggcccatg
 +
</pre>
  
 
===References===
 
===References===

Latest revision as of 05:26, 16 July 2008

{R6K} conditional origin of replication in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR ca1182F/ca1182R on pG80 CRIM plasmid  (BglII/XhoI)
Sub into pBca9145-Bca1144#5               (BglII/XhoI,L)
Product is pBca9145-Bca1182
----
ca1182F	BBb Biobricking of R6K
ctctgAGATCTaGCAGTTCAACCTGTTGATAG
ca1182R	BBb biobricking of R6K
gcaaaCTCGAGttaGGATCCgtgattcgcacgggcccatg

References