Difference between revisions of "Part:BBa K2560106:Design"

 
 
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===Design Notes===
 
===Design Notes===
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More information coming soon!
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<html>
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<p align="justify">
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This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our <a href="http://2018.igem.org/Team:Marburg/Design">Design Page</a>.
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</html>
 
===Source===
 
===Source===
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<html>
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The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this
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<a href="https://parts.igem.org/Help:Promoters/Construction">site</a>.
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</html>
  
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More information coming soon!
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<b> Forward Oligo:</b>
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CTCGCGCTTGTTGGAGACGAGCT
  
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===References===
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<b> Reverse Oligo:</b>
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CTCAAGCTCGTCTCCAACAAGCG

Latest revision as of 00:43, 17 October 2018


Phytobrick version of 3'Con1_inv_Res Connector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our Design Page.

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGCGCTTGTTGGAGACGAGCT

Reverse Oligo: CTCAAGCTCGTCTCCAACAAGCG