Difference between revisions of "Part:BBa J72003"

Latest revision as of 05:23, 16 July 2008

{R6K} conditional origin of replication in BBb

The R6K origin of replication requires a protein called pi encoded by the pir gene for function. At higher expression of pir in strains containing the pir116 allele, R6K plasmids replicate at high copy comparable to pUC plasmids. At lower expression levels such as observed in pir+ strains, they express at medium copy comparable to p15A plasmids. For most laboratory E. coli strains, there is no pir gene, and no replication is observed. In house, this part is referred to as pBca9145-Bca1182 {R6k}.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_J72003 short</partinfo>
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+The R6K origin of replication requires a protein called pi encoded by the pir gene for function.  At higher expression of pir in strains containing the pir116 allele, R6K plasmids replicate at high copy comparable to pUC plasmids.  At lower expression levels such as observed in pir+ strains, they express at medium copy comparable to p15A plasmids.  For most laboratory E. coli strains, there is no pir gene, and no replication is observed.  In house, this part is referred to as pBca9145-Bca1182 {R6k}.
+----
+This part is in BBb Format.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BBb Format is available at:<br>
+[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
 <!-- Add more about the biology of this part here