Difference between revisions of "Part:BBa K2560075:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | <html> | |
− | + | <p align="justify"> | |
− | + | This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our <a href="http://2018.igem.org/Team:Marburg/Design">Design Page</a>. | |
− | + | </html> | |
===Source=== | ===Source=== | ||
+ | <html> | ||
+ | The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this | ||
+ | <a href="https://parts.igem.org/Help:Promoters/Construction">site</a>. | ||
+ | </html> | ||
− | + | <b> Forward Oligo:</b> | |
+ | CTCGCGCTTACTGGAGACGAGCT | ||
− | + | <b> Reverse Oligo:</b> | |
+ | CTCAAGCTCGTCTCCAGTAAGCG |
Revision as of 00:17, 17 October 2018
Phytobrick version of 5'Con1_L1 Short Res Connector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our Design Page.
Source
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.
Forward Oligo: CTCGCGCTTACTGGAGACGAGCT
Reverse Oligo: CTCAAGCTCGTCTCCAGTAAGCG