Difference between revisions of "Part:BBa J72002:Design"
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<partinfo>BBa_J72002 short</partinfo> | <partinfo>BBa_J72002 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | + | <pre> | |
| − | + | PCR ca1121R/ca1172R on pBca1121 (920bp, BglII/XhoI) | |
| + | Sub into pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) | ||
| + | Product is pBca9145-Bca1172 | ||
| + | ---- | ||
| + | ca1121R EcoRI/BglII cmR cassette | ||
| + | gcaatAGATCTttgatcgggcacgtaagagg | ||
| + | ca1172R Biobrickin CmR extreme basic part | ||
| + | gcaaaCTCGAGttaGGATCCgaggcttggattctcacc | ||
| + | </pre> | ||
===References=== | ===References=== | ||
Latest revision as of 05:17, 16 July 2008
{CmR} Chloramphenicol resistance gene in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
PCR ca1121R/ca1172R on pBca1121 (920bp, BglII/XhoI) Sub into pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) Product is pBca9145-Bca1172 ---- ca1121R EcoRI/BglII cmR cassette gcaatAGATCTttgatcgggcacgtaagagg ca1172R Biobrickin CmR extreme basic part gcaaaCTCGAGttaGGATCCgaggcttggattctcacc