Difference between revisions of "Part:BBa K2680270:Design"

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===Design Notes===
 
===Design Notes===
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Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary
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Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary
  
 
===Source===
 
===Source===
  
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Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology.
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deCFP is a destabilized enhanced variant of CFP, which originates from GFP, a protein found in <I>Aequorea victoria</I>.
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===References===
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Latest revision as of 00:09, 17 October 2018


3G deCFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 738


Design Notes

Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary

Source

deCFP is a destabilized enhanced variant of CFP, which originates from GFP, a protein found in Aequorea victoria.