Difference between revisions of "Part:BBa J72001:Design"
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<partinfo>BBa_J72001 short</partinfo> | <partinfo>BBa_J72001 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | + | <pre> | |
| − | + | Overlap extension of ca1090F and ca1115R (71bp, EcoRI/BamHI) | |
| − | + | sub into pBca9145-Bca1089 (EcoRI/BamHI, 2063+696, L) | |
| + | Product is pBca9145-Bca1115 | ||
| + | ---- | ||
| + | ca1090F Forward EcoRI/BglII wobble for RBS part | ||
| + | gatttctggaattcatgAGATCT | ||
| + | ca1115R FRT basic BBb part by wobble | ||
| + | cttgcggatccgaagttcctattctctagaaagtataggaacttcgcAGATCTcatgaattccagaa | ||
| + | </pre> | ||
===References=== | ===References=== | ||
Latest revision as of 05:11, 16 July 2008
{FRT} recombination site for flp recombinase in BBb
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 18
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 18
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 18
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
Overlap extension of ca1090F and ca1115R (71bp, EcoRI/BamHI) sub into pBca9145-Bca1089 (EcoRI/BamHI, 2063+696, L) Product is pBca9145-Bca1115 ---- ca1090F Forward EcoRI/BglII wobble for RBS part gatttctggaattcatgAGATCT ca1115R FRT basic BBb part by wobble cttgcggatccgaagttcctattctctagaaagtataggaacttcgcAGATCTcatgaattccagaa