Difference between revisions of "Part:BBa K2560070:Design"
(→Design Notes) |
(→Design Notes) |
||
Line 11: | Line 11: | ||
<p align="justify"> | <p align="justify"> | ||
− | This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our <a href="http://2018.igem.org/Team:Marburg/Design">Design Page</a> | + | This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our <a href="http://2018.igem.org/Team:Marburg/Design">Design Page</a>. |
</html> | </html> | ||
Latest revision as of 23:54, 16 October 2018
Phytobrick version of 3'Con1_L1 Connector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our Design Page.
Source
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.
Forward Oligo: CTCGCGCTTACTGGAGACGAGCT
Reverse Oligo: CTCAAGCTCGTCTCCAGTAAGCG