Difference between revisions of "Part:BBa K1469005"
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<partinfo>BBa_K1469005 parameters</partinfo>
 
<partinfo>BBa_K1469005 parameters</partinfo>
 
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=====Improvement=====
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2018 SSTi-SZGD coexpress tuaD and gtaB together, because gtab and tuad are two of the genes involved in the synthesis of UDP-glucose dehydrogenase and UDP-gulcose pyrophosphorylase. The results showed that co-expressing of tuaD and gtaB genes increased HA accumulation, suggesting that UDP-glucose dehydrogenase and UDP-gulcose pyrophosphorylase, which commit to the biosynthesis of one of the HA precursors, are rate-limiting enzymes in HA biosynthesis.

Revision as of 23:17, 16 October 2018

gtaB

UTP--glucose-1-phosphate uridylyltransferase of Bacillus megaterium. The enzyme catalyzes the conversion of glucose 1-phospahate to UDP-glucose 1-phosphate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Improvement

2018 SSTi-SZGD coexpress tuaD and gtaB together, because gtab and tuad are two of the genes involved in the synthesis of UDP-glucose dehydrogenase and UDP-gulcose pyrophosphorylase. The results showed that co-expressing of tuaD and gtaB genes increased HA accumulation, suggesting that UDP-glucose dehydrogenase and UDP-gulcose pyrophosphorylase, which commit to the biosynthesis of one of the HA precursors, are rate-limiting enzymes in HA biosynthesis.