Difference between revisions of "Part:BBa K108014:Experience"

 
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<I>Edinburgh OG 2018</I>
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We access the online NCBI data (NCBI: AM260479.1) and use reference 1 as guideline and found out the current available the functional promoter is being 134bp shorter. Therefore we have used the new phaR and promoter sequence as our new template. Due to the excessive GC in the sequence, we have it codon optimised to E.coli and generated the part through the IDT company service.
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Reference 1. Yamada M, Takahashi S, Okahata Y, Doi Y, Numata K. (2013 ) Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express. 3(1):6.
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Revision as of 23:10, 16 October 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K108014

User Reviews

UNIQ95a38dd98a1f9b7b-partinfo-00000000-QINU UNIQ95a38dd98a1f9b7b-partinfo-00000001-QINU

•

Edinburgh OG 2018

We access the online NCBI data (NCBI: AM260479.1) and use reference 1 as guideline and found out the current available the functional promoter is being 134bp shorter. Therefore we have used the new phaR and promoter sequence as our new template. Due to the excessive GC in the sequence, we have it codon optimised to E.coli and generated the part through the IDT company service.

Reference 1. Yamada M, Takahashi S, Okahata Y, Doi Y, Numata K. (2013 ) Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express. 3(1):6.

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