Difference between revisions of "Part:BBa K108015:Experience"

 
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===Applications of BBa_K108015===
 
===Applications of BBa_K108015===
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<partinfo>BBa_K1149051 AddReview 5</partinfo>
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<I>Edinburgh OG 2018</I>
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We access the online NCBI data and found out the current available phaR sequence (AM260479.1) is being 4 bp different to BBa_K108015. Therefore we have used the new phaR and promoter sequence as our new template. Due to the excessive GC in the sequence, we have it codon optimised to E.coli and generated the part through the IDT company service.
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https://static.igem.org/mediawiki/parts/c/cd/T--Edinburgh_OG_BBa_K2739000--image_9.jpeg
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Figure 1. Comparison of phaR sequence from  iGEM08_Tsinghua and online NCBI. Capital letters: NCBI and Small letter: iGEM08_Tsinghua.
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https://static.igem.org/mediawiki/parts/9/9a/T--Edinburgh_OG_BBa_K2739000--image_10.jpeg
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Figure 2. Comparison of phaR sequence before and after codon optimisation. Capital letters: NCBI and Small letter: codon optimised version.
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===User Reviews===
 
===User Reviews===

Revision as of 23:06, 16 October 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K108015

•••••

Edinburgh OG 2018

We access the online NCBI data and found out the current available phaR sequence (AM260479.1) is being 4 bp different to BBa_K108015. Therefore we have used the new phaR and promoter sequence as our new template. Due to the excessive GC in the sequence, we have it codon optimised to E.coli and generated the part through the IDT company service.


T--Edinburgh_OG_BBa_K2739000--image_9.jpeg

Figure 1. Comparison of phaR sequence from iGEM08_Tsinghua and online NCBI. Capital letters: NCBI and Small letter: iGEM08_Tsinghua.

T--Edinburgh_OG_BBa_K2739000--image_10.jpeg

Figure 2. Comparison of phaR sequence before and after codon optimisation. Capital letters: NCBI and Small letter: codon optimised version.

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User Reviews

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