Difference between revisions of "Part:BBa K2765021:Design"
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Fig.4 The size of yno1 (1713bp)
 
Fig.4 The size of yno1 (1713bp)
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https://static.igem.org/mediawiki/2018/b/b3/T--BIT-China--PartRegulator_yno1_Fig5_Plasmid.png
 
https://static.igem.org/mediawiki/2018/b/b3/T--BIT-China--PartRegulator_yno1_Fig5_Plasmid.png
  
 
Fig.5 plasmid of yno1 expression
 
Fig.5 plasmid of yno1 expression
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https://static.igem.org/mediawiki/2018/5/5e/T--BIT-China--PartRegulator_yno1_Fig6_ColonyPCR.png
 
https://static.igem.org/mediawiki/2018/5/5e/T--BIT-China--PartRegulator_yno1_Fig6_ColonyPCR.png

Revision as of 22:55, 16 October 2018

To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by gal1 promoter, and thus the target gene is induced by galactose and repressed by glucose.

T--BIT-China--PartRegulator_yno1_Fig4_Size.png

Fig.4 The size of yno1 (1713bp)


T--BIT-China--PartRegulator_yno1_Fig5_Plasmid.png

Fig.5 plasmid of yno1 expression


T--BIT-China--PartRegulator_yno1_Fig6_ColonyPCR.png

Fig.6 result of Colony PCR

1~7: negative results

8: positive result (yno1 1713bp)