Difference between revisions of "Part:BBa K2765021:Design"
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https://static.igem.org/mediawiki/2018/8/88/T--BIT-China--PartRegulator_yno1_Fig2_Overexpress.png
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To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by gal1 promoter, and thus the target gene is induced by galactose and repressed by glucose.
  
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Fig.2 overexpress yno1
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https://static.igem.org/mediawiki/2018/7/7b/T--BIT-China--PartRegulator_yno1_Fig4_Size.png
  
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https://static.igem.org/mediawiki/2018/a/ae/T--BIT-China--PartRegulator_yno1_Fig3_process_of_Electron_transformation.png
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Fig.4 The size of yno1 (1713bp)
  
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https://static.igem.org/mediawiki/2018/b/b3/T--BIT-China--PartRegulator_yno1_Fig5_Plasmid.png
  
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Fig.5 plasmid of yno1 expression
  
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Fig. 3 process of Electron transformation
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https://static.igem.org/mediawiki/2018/5/5e/T--BIT-China--PartRegulator_yno1_Fig6_ColonyPCR.png
  
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Overexpression yno1 gene can causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
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Fig.6 result of Colony PCR
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1~7: negative results
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8: positive result (yno1 1713bp)

Revision as of 22:54, 16 October 2018

To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by gal1 promoter, and thus the target gene is induced by galactose and repressed by glucose.

T--BIT-China--PartRegulator_yno1_Fig4_Size.png

Fig.4 The size of yno1 (1713bp)

T--BIT-China--PartRegulator_yno1_Fig5_Plasmid.png

Fig.5 plasmid of yno1 expression

T--BIT-China--PartRegulator_yno1_Fig6_ColonyPCR.png

Fig.6 result of Colony PCR

1~7: negative results

8: positive result (yno1 1713bp)