Difference between revisions of "Part:BBa K2560030:Design"

(Design Notes)
(References)
 
Line 25: Line 25:
 
<b> Reverse Oligo:</b>
 
<b> Reverse Oligo:</b>
 
CTCAAGTAGCTAGCACAATACCTAGGACTGAGCTAGCCGTCAACTCC
 
CTCAAGTAGCTAGCACAATACCTAGGACTGAGCTAGCCGTCAACTCC
 
===References===
 

Latest revision as of 22:41, 16 October 2018


Phytobrick version of BBa_J23118


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was obtained from part BBa_J23118. The parts sequence was not changed apart from adding the promoter overhangs that are required for subsequent cloning.

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTATTGTGCTAGCTACT

Reverse Oligo: CTCAAGTAGCTAGCACAATACCTAGGACTGAGCTAGCCGTCAACTCC