Difference between revisions of "Part:BBa K2560030:Design"
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<b> Reverse Oligo:</b> | <b> Reverse Oligo:</b> | ||
CTCAAGTAGCTAGCACAATACCTAGGACTGAGCTAGCCGTCAACTCC | CTCAAGTAGCTAGCACAATACCTAGGACTGAGCTAGCCGTCAACTCC | ||
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Latest revision as of 22:41, 16 October 2018
Phytobrick version of BBa_J23118
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was obtained from part BBa_J23118. The parts sequence was not changed apart from adding the promoter overhangs that are required for subsequent cloning.
Source
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.
Forward Oligo: CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTATTGTGCTAGCTACT
Reverse Oligo: CTCAAGTAGCTAGCACAATACCTAGGACTGAGCTAGCCGTCAACTCC