Difference between revisions of "Part:BBa K137017"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K137017 short</partinfo>
 
<partinfo>BBa_K137017 short</partinfo>
  
−
Galactose oxidase is a fungal enzyme that uses molecular oxygen to oxidizes galactose and several other primary alcohols to the corresponding aldehyde, while also producing hydrogen peroxide. This particular galaxtose oxidase is not wild type. It has been optimized through directed evolution by the Arnold lab at CalTech to be functionally expressed in vivo in E. coli and to be thermostable. (Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 2001 Sep; 14(9) 699-704. pmid:11707617). This parts has undergone two rounds of site directed mutagenesis to remove one EcoRI and one PstI site. In our hands, it is functional towards galactose in vivo.
+
Galactose oxidase is a fungal enzyme that uses molecular oxygen to oxidizes galactose and several other primary alcohols to the corresponding aldehyde, while also producing hydrogen peroxide. This particular galaxtose oxidase is not wild type. It has been optimized through directed evolution by the Arnold lab at CalTech to be functionally expressed in vivo in E. coli and to be thermostable. (Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 2001 Sep; 14(9) 699-704. pmid:11707617). This part has undergone two rounds of site directed mutagenesis to remove one EcoRI and one PstI site. In our hands, it is functional towards galactose in vivo.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:51, 15 July 2008

Galactose Oxidase

Galactose oxidase is a fungal enzyme that uses molecular oxygen to oxidizes galactose and several other primary alcohols to the corresponding aldehyde, while also producing hydrogen peroxide. This particular galaxtose oxidase is not wild type. It has been optimized through directed evolution by the Arnold lab at CalTech to be functionally expressed in vivo in E. coli and to be thermostable. (Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 2001 Sep; 14(9) 699-704. pmid:11707617). This part has undergone two rounds of site directed mutagenesis to remove one EcoRI and one PstI site. In our hands, it is functional towards galactose in vivo.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1294
    Illegal NotI site found at 517
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1424
    Illegal BamHI site found at 144
    Illegal BamHI site found at 589
    Illegal BamHI site found at 624
    Illegal BamHI site found at 782
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]