Difference between revisions of "Part:BBa K209023"

Revision as of 21:15, 16 October 2018

Aar1 C-D part, FKBP

dimerizes with FRB when rapamycin is present

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

2018 Peking Team improvement

Team Peking 2018 improved this part.

Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope.

https://parts.igem.org/Part:BBa_K2601008

Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601025 https://parts.igem.org/Part:BBa_K2601026 https://parts.igem.org/Part:BBa_K2601027

Moreover, we fused FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.

https://parts.igem.org/Part:BBa_K2601011

Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry.

https://parts.igem.org/Part:BBa_K2601037 https://parts.igem.org/Part:BBa_K2601038

@@ Line 21: / Line 21: @@
 Team Peking 2018 improved this part.
-First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope.
+Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope.
 https://parts.igem.org/Part:BBa_K2601008
-Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
+Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.
 https://parts.igem.org/Part:BBa_K2601025
@@ Line 31: / Line 31: @@
 https://parts.igem.org/Part:BBa_K2601027
-What's more, we fuse FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
+Moreover, we fused FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
 https://parts.igem.org/Part:BBa_K2601011
-Then, we drive the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
+Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry.
 https://parts.igem.org/Part:BBa_K2601037
 https://parts.igem.org/Part:BBa_K2601038