Difference between revisions of "Part:BBa K2637049:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We added the constitutive <i>TDH3</i> promoter and <i>ADH1</i> terminator to the [https://parts.igem.org/Part:BBa_E2030 Part:BBa_E2030] to make the cassette which can express EYFP continously | + | We added the constitutive <i>TDH3</i> promoter and <i>ADH1</i> terminator to the [https://parts.igem.org/Part:BBa_E2030 Part:BBa_E2030] to make the cassette which can express EYFP continously. |
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===Source=== | ===Source=== | ||
Latest revision as of 21:00, 16 October 2018
EYFP cassette with TDH3 Promoter and ADH1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 366
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1717
Design Notes
We added the constitutive TDH3 promoter and ADH1 terminator to the Part:BBa_E2030 to make the cassette which can express EYFP continously.
Source
The gene EYFP derives from Part:BBa_E2030. The TDH3 promoter and ADH1 terminator are from saccharomyces cerevisiae.