Difference between revisions of "Part:BBa K2637048:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We connected <i>Fluc</i> with constitutive <i>TDH3</i> promoter and <i>ADH1</i> terminator to make the cassette which can express Fluc continuously.
+
We connected <i>Fluc</i> witth the constitutive <i>TDH3</i> promoter and <i>ADH1</i> terminator to make the cassette which can express Fluc continuously.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The gene <i>NanoLuc</i> derives from [https://parts.igem.org/Part:BBa_K1680009 Part:BBa_K1680009]. The <i>TDH3</i> promoter and <i>ADH1</i> terminator are from <i>saccharomyces cerevisiae</i>.
+
The sequence of <i>Fluc</i> is already published by Promega [http://www.promega.com/vectors/pgl4.10.txt here]. The <i>TDH3</i> promoter and <i>ADH1</i> terminator are from <i>saccharomyces cerevisiae</i>.
  
  
 
===References===
 
===References===

Latest revision as of 20:48, 16 October 2018


Fluc with TDH3 Promoter and ADH1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1119
    Illegal NgoMIV site found at 2463
    Illegal NgoMIV site found at 2484
    Illegal AgeI site found at 2187
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2369


Design Notes

We connected Fluc witth the constitutive TDH3 promoter and ADH1 terminator to make the cassette which can express Fluc continuously.


Source

The sequence of Fluc is already published by Promega [http://www.promega.com/vectors/pgl4.10.txt here]. The TDH3 promoter and ADH1 terminator are from saccharomyces cerevisiae.


References