Difference between revisions of "Part:BBa K2739008:Design"
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| − | The sequence of present Bktb is isolated from Ralstonia Eutropha and codon optimised to E.coli. The original sequence would be accessed in NCBI: NC_015726.1 | + | The sequence of present Bktb is isolated from Ralstonia Eutropha and codon optimised to E.coli. The original sequence would be accessed in NCBI: NC_015726.1. |
The sequence is codon optimised by IDT. | The sequence is codon optimised by IDT. | ||
Revision as of 20:20, 16 October 2018
Bktb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 831
Design Notes
The length of the Bktb is 1185bp and excess the 1kb length limit of the IDT sponsor limitation to be generated. Therefore two overlapped fragments of Bktb were then designed and generated by IDT. A set of PCR primer were then design to generate the whole Bktb through the PCR amplification.
Source
The sequence of present Bktb is isolated from Ralstonia Eutropha and codon optimised to E.coli. The original sequence would be accessed in NCBI: NC_015726.1.
The sequence is codon optimised by IDT.
References
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