Difference between revisions of "Part:BBa K2739008:Design"
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===Source===
 
===Source===
  
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The sequence of present Bktb is isolated from Ralstonia Eutropha and codon optimised to E.coli. The original sequence would be accessed in NCBI: https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?from=1485802&to=1486986&report=fasta
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The sequence of present Bktb is isolated from Ralstonia Eutropha and codon optimised to E.coli. The original sequence would be accessed in NCBI: NC_015726.1
  
 
===References===
 
===References===
  
 
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Revision as of 20:19, 16 October 2018


Bktb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 831


Design Notes

The length of the Bktb is 1185bp and excess the 1kb length limit of the IDT sponsor limitation to be generated. Therefore two overlapped fragments of Bktb were then designed and generated by IDT. A set of PCR primer were then design to generate the whole Bktb through the PCR amplification.


Source

The sequence of present Bktb is isolated from Ralstonia Eutropha and codon optimised to E.coli. The original sequence would be accessed in NCBI: NC_015726.1

References

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