Difference between revisions of "Part:BBa K2739004:Design"
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===Source===
 
===Source===
  
−
The phasin basic part and sequence information were obtained from the Composite part of BBa_K390501.  
+
The phasin basic part and sequence information were obtained from the Composite part of BBa_K390501. (And Phasin sequence optimised by previous team)
−
 
+
−
Phasin sequence codon optimizied through IDT
+
  
 
===References===
 
===References===
  
 
1. Yamada M, Takahashi S, Okahata Y, Doi Y, Numata K. (2013 ) Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express. 3(1):6.
 
1. Yamada M, Takahashi S, Okahata Y, Doi Y, Numata K. (2013 ) Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express. 3(1):6.

Revision as of 20:17, 16 October 2018


Phasin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Available Phasin biobrick (BBa_K208001) are found in Lac promoter-Phasin-HlyA biobrick, BBa_K390501. Through sequencing, it was found to contain a stop codon. A set of PCR primers was designed and used to remove the stop codon. In the primer design, a HindIII restriction enzyme site, which is not present in the original biobrick, was introduced and allow the confirmation of the success stop codon removal.


Source

The phasin basic part and sequence information were obtained from the Composite part of BBa_K390501. (And Phasin sequence optimised by previous team)

References

1. Yamada M, Takahashi S, Okahata Y, Doi Y, Numata K. (2013 ) Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express. 3(1):6.