Difference between revisions of "Part:BBa K209496"

 
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<partinfo>BBa_K209496 parameters</partinfo>
 
<partinfo>BBa_K209496 parameters</partinfo>
 
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===2018 Peking Team improvement===
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Team Peking 2018 improved this part.
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First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope.
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https://parts.igem.org/Part:BBa_K2601007
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Then, we drive the expression of Frb-yEGFP with 3 promoters pTet07 and PDH3. The expression of these promoters were measure by flow cytometry.
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https://parts.igem.org/Part:BBa_K2601021
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https://parts.igem.org/Part:BBa_K2601023
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What's more, we fuse Frb-yEGFP with HOtag6. This part can form phase separation with FKBP-yEGFP-HOTag3 in the presence of rapamycin.
 +
 +
https://parts.igem.org/Part:BBa_K2601010
 +
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Then, we drive the expression of Frb-yEGFP-HOTag6 with 2 promoters pTet07, pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
 +
 +
https://parts.igem.org/Part:BBa_K2601032
 +
https://parts.igem.org/Part:BBa_K2601033
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https://parts.igem.org/Part:BBa_K2601034

Revision as of 20:03, 16 October 2018


AarI B-D part, FRB



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



2018 Peking Team improvement

Team Peking 2018 improved this part.

First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope.

https://parts.igem.org/Part:BBa_K2601007

Then, we drive the expression of Frb-yEGFP with 3 promoters pTet07 and PDH3. The expression of these promoters were measure by flow cytometry.

https://parts.igem.org/Part:BBa_K2601021 https://parts.igem.org/Part:BBa_K2601023


What's more, we fuse Frb-yEGFP with HOtag6. This part can form phase separation with FKBP-yEGFP-HOTag3 in the presence of rapamycin.

https://parts.igem.org/Part:BBa_K2601010

Then, we drive the expression of Frb-yEGFP-HOTag6 with 2 promoters pTet07, pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.

https://parts.igem.org/Part:BBa_K2601032 https://parts.igem.org/Part:BBa_K2601033 https://parts.igem.org/Part:BBa_K2601034