Difference between revisions of "Part:BBa K2680269:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
− | + | Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology. | |
===References=== | ===References=== |
Latest revision as of 16:29, 16 October 2018
3G cre Recombinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 96
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 47
Illegal BsaI.rc site found at 1094
Design Notes
Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.
Source
Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology.