Difference between revisions of "Part:BBa K2552007"
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This part is used to regulate azurin(14kDa) expression by Pyear promoter. We add the His tag (5 Histidine amino acids) at the end of azurin protein in order to exam the expression of it. We insert the Pyear promoter and azurin protein into a regulative vector pCDFDuet1. After inducing with sedium nitrate, we extract all the protein in our strain and exam it by Western Blotting method. From the Fig1, we demonstrate that azurin protein successfully express with the His tag. | This part is used to regulate azurin(14kDa) expression by Pyear promoter. We add the His tag (5 Histidine amino acids) at the end of azurin protein in order to exam the expression of it. We insert the Pyear promoter and azurin protein into a regulative vector pCDFDuet1. After inducing with sedium nitrate, we extract all the protein in our strain and exam it by Western Blotting method. From the Fig1, we demonstrate that azurin protein successfully express with the His tag. | ||
<p>https://parts.igem.org/File:T--SJTU-BioX-Shanghai--WesternBlotting.jpg<p/> | <p>https://parts.igem.org/File:T--SJTU-BioX-Shanghai--WesternBlotting.jpg<p/> | ||
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| + | <div class="img_in_text zoom_out_able"> | ||
| + | <img src="https://parts.igem.org/File:T--SJTU-BioX-Shanghai--WesternBlotting.jpg"/> | ||
| + | |||
| + | </div> | ||
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Revision as of 16:06, 16 October 2018
Pyear-azurin
This part is used to regulate azurin expression by Pyear promoter.
Usage and Biology
This part is used to regulate azurin(14kDa) expression by Pyear promoter. We add the His tag (5 Histidine amino acids) at the end of azurin protein in order to exam the expression of it. We insert the Pyear promoter and azurin protein into a regulative vector pCDFDuet1. After inducing with sedium nitrate, we extract all the protein in our strain and exam it by Western Blotting method. From the Fig1, we demonstrate that azurin protein successfully express with the His tag.
https://parts.igem.org/File:T--SJTU-BioX-Shanghai--WesternBlotting.jpg<p/>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]