Difference between revisions of "Part:BBa K2817013"
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Figure 1. The fluorescence of overnight bacterial suspension after centrifugation at 3000 rpm for 5 min. | Figure 1. The fluorescence of overnight bacterial suspension after centrifugation at 3000 rpm for 5 min. | ||
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| + | [1] Zhang G, Brokx S, Weiner JH (2006) Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nat Biotechnol 24:100–104 | ||
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Revision as of 16:05, 16 October 2018
J23100-RBS-YebF-GFP
YebF is a secretion protein from E.coli. It’s used as secretion tag.We combine YebF with GFP to characterize its performance.
Usage and Biology
When testing the validity of the secretory tag YebF, we transformed the constructed YebF-GFP plasmid into DH5α and cultured overnight at 37 ℃. The supernatant was centrifuged at 3000 rpm for 5 min, and the supernatant was taken for fluorescence detection. The results are shown in the Figure 1. But we forgot to set up a positive control group. Due to time constraints, we have no time to repeat this experiment. But as a secretory tag used in both the literature and the iGEM team, we believe it can actually work.
Figure 1. The fluorescence of overnight bacterial suspension after centrifugation at 3000 rpm for 5 min.
[1] Zhang G, Brokx S, Weiner JH (2006) Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nat Biotechnol 24:100–104
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1056