Difference between revisions of "Part:BBa K2570019"

Revision as of 15:33, 16 October 2018

tetR+GFP+mazF+PLtetO1+RFP

The Tet-Off system which turns expression of its controlling genes OFF by the addition of tetracycline and its derivative is often used for this purpose and has been applied to several organisms.

The figure shows a strategy for metabolic state switching module using a synthetic metabolic regulator.

Fig. (A) Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode. (B) Design of the synthetic metabolic regulator.

AraC repressor inhibits transcription from the PBAD promoter. When transcription by the PBAD promoter is induced with arabinose addition, expression of tetR and GFP is promoted and the expression of mCherry under the PLtetO1 promoter is inhibited by the TetR repressor. Without arabinose addition, expression of tetR and GFP under the PBAD promoter is inhibited by the araC repressor and the expression of mCherry under the PLtetO1 promoter is promoted.

Figure.(C) Fluorescence levels per OD at different addition of anhydrotetracycline (ATc).

Figure.(D) Fluorescence images at different addition of anhydrotetracycline (ATc).

anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.

Sequence and Features