Difference between revisions of "Part:BBa K2570019:Design"

 
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We have added an enzyme cutting site in the design to facilitate BIOBRICK.
 
We have added an enzyme cutting site in the design to facilitate BIOBRICK.
  
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+
anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.
  
 
===Source===
 
===Source===

Revision as of 15:32, 16 October 2018


tetR+GFP+mazF+PLtetO1+RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2590
    Illegal AgeI site found at 2702
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have added an enzyme cutting site in the design to facilitate BIOBRICK.

anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.

Source

We ordered the part DNA from a synthesis company and had codon optimization of the sequence.

References