Difference between revisions of "Part:BBa K2729010"
Line 3: Line 3:
 
<partinfo>BBa_K2729010 short</partinfo>
 
<partinfo>BBa_K2729010 short</partinfo>
  
 +
==Usage and Biology==
 
Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
 
Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
  
−
<!-- Add more about the biology of this part here
+
==Characterization==
−
===Usage and Biology===
+
The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.
  
−
<!-- -->
+
==Sequence and Features==
−
<span class='h3bb'>Sequence and Features</span>
+
 
<partinfo>BBa_K2729010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2729010 SequenceAndFeatures</partinfo>
−
 
−
 
−
<!-- Uncomment this to enable Functional Parameter display
 
−
===Functional Parameters===
 
−
<partinfo>BBa_K2729010 parameters</partinfo>
 
−
<!-- -->
 
−
 
−
==Characterization==
 
−
The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.
 
  
 
==reference==
 
==reference==
−
- [https://pubs.acs.org/doi/10.1021/bi500724k Nemésio H, Villalaín J. Membranotropic regions of the dengue virus prM protein. Biochemistry. 2014 Aug 19;53(32):5280-9. doi: 10.1021/bi500724k. Epub 2014 Aug 6. PubMed PMID: 25076193.]
+
- [https://pubs.acs.org/doi/10.1021/bi500724k Membranotropic regions of the dengue virus prM protein]

Revision as of 14:33, 16 October 2018


DENV1 ancC-prM-E (Dengue Virus Serotype I)

Usage and Biology

Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1831
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1831
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1245
    Illegal BglII site found at 1856
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1831
    Illegal AgeI site found at 1450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 217
    Illegal BsaI.rc site found at 367

reference

- Membranotropic regions of the dengue virus prM protein