Difference between revisions of "Part:BBa K2729006"
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Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.
 
Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2729006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2729006 SequenceAndFeatures</partinfo>
  

Revision as of 14:25, 16 October 2018


DENV1 ancC-prM-E (Dengue Virus Serotype I)

Usage and Biology

EGFP contains chromophore mutations that make the protein 35 times brighter than wild-type GFP, and is codon-optimized for higher expression in mammalian cells. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.

Characterization

EGFP is derived from the jellyfish Aequorea victoria and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the qualitative experiment - description comes here.


As shown in Figure 1, the band comes around 1,500 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

Characterization

EGFPは、