Difference between revisions of "Part:BBa K2729006"
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<partinfo>BBa_K2729006 short</partinfo>
 
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===Usage and Biology===
  
 
EGFP contains chromophore mutations that make the protein 35 times brighter than wild-type GFP, and is codon-optimized for higher expression in mammalian cells.
 
EGFP contains chromophore mutations that make the protein 35 times brighter than wild-type GFP, and is codon-optimized for higher expression in mammalian cells.
 
FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.
 
FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.
  
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===Characterization===
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===Usage and Biology===
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EGFP is derived from <i>the jellyfish Aequorea victoria</i> and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
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As shown in Figure 1, the band comes around 1,500 bp.
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===Analysis===
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Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.
  
 
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<partinfo>BBa_K2729006 SequenceAndFeatures</partinfo>
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2729006 parameters</partinfo>
 
<partinfo>BBa_K2729006 parameters</partinfo>

Revision as of 14:24, 16 October 2018


DENV1 ancC-prM-E (Dengue Virus Serotype I)

Usage and Biology

EGFP contains chromophore mutations that make the protein 35 times brighter than wild-type GFP, and is codon-optimized for higher expression in mammalian cells. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.

Characterization

EGFP is derived from the jellyfish Aequorea victoria and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.


As shown in Figure 1, the band comes around 1,500 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

Characterization

EGFPは、