Difference between revisions of "Part:BBa K2729005"
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<partinfo>BBa_K2729005 short</partinfo> | <partinfo>BBa_K2729005 short</partinfo> | ||
+ | ===Usage and Biology=== | ||
Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. | Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. | ||
− | + | ===Characterization=== | |
− | === | + | |
− | + | The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it. | |
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+ | [[File:T--Tokyo_Tech--test-electrop.gif|thumb|left|300px| '''Figure 1:''' '''Result of the qualitative experiment''' - description comes here. ]] | ||
+ | |||
+ | <br> | ||
+ | As shown in Figure 1, the band of DENV4 E come around 1,500 bp. | ||
+ | <br style="clear: both" /> | ||
+ | |||
+ | ===Analysis=== | ||
+ | |||
+ | Our team successfully validated that the sequence of DENV4 E was synthesized, and obtained enough amount of it for the transduction. | ||
+ | |||
+ | ===Sequence and Features=== | ||
+ | <partinfo>BBa_K2729005 SequenceAndFeatures</partinfo> | ||
− | + | ===Reference=== | |
− | === | + | - [https://www.ncbi.nlm.nih.gov/gene/507572 NCBI Gene Database] |
− | + | ||
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Revision as of 14:20, 16 October 2018
DENV4 E (Envelope Protein | Dengue Virus Serotype IV)
Usage and Biology
Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
Characterization
The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
As shown in Figure 1, the band of DENV4 E come around 1,500 bp.
Analysis
Our team successfully validated that the sequence of DENV4 E was synthesized, and obtained enough amount of it for the transduction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1249