Difference between revisions of "Part:BBa K2548012"
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| − | This | + | This design is used to synthesize and extract distinct SpALF4 in order to test its effectiveness. We chose the pTac promoter, a hybrid of Lac and Trp promoters, to induce SpALF4 production in E. coli because it is the most accessible promoter in our lab. The promoter can be induced by IPTG to remove the repression on transcription from LacI. We also add 6xHis-tag after sequences of SpALF4 to extract it out with the nickel column. |
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Revision as of 14:13, 16 October 2018
LacI - pTac - SpALF4 + 6xHis Tag - Terminator
This design is used to synthesize and extract distinct SpALF4 in order to test its effectiveness. We chose the pTac promoter, a hybrid of Lac and Trp promoters, to induce SpALF4 production in E. coli because it is the most accessible promoter in our lab. The promoter can be induced by IPTG to remove the repression on transcription from LacI. We also add 6xHis-tag after sequences of SpALF4 to extract it out with the nickel column.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]