Difference between revisions of "Part:BBa K2587030:Design"
(→Design Notes) |
(→Source) |
||
| Line 11: | Line 11: | ||
===Source=== | ===Source=== | ||
| − | Ddh was isolated from the genome of Corynebacterium glutamicum. The other parts were taken from the CIDAR MoClo toolbox | + | <i>Ddh</i> was isolated from the genome of <i>Corynebacterium glutamicum</i>. The other parts were taken from the CIDAR MoClo toolbox |
===References=== | ===References=== | ||
Latest revision as of 14:08, 16 October 2018
Pj23106_RBS_ddh_T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 422
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 390
Illegal NgoMIV site found at 615
Illegal AgeI site found at 825 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We performed a side directed mutagenesis of the recognition sites of the restriction enzymes BsaI and BbsI in the coding sequence of ddh.
Source
Ddh was isolated from the genome of Corynebacterium glutamicum. The other parts were taken from the CIDAR MoClo toolbox