Difference between revisions of "Part:BBa K2729000"
| Line 4: | Line 4: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | |||
| − | |||
| − | |||
Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. | Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. | ||
| − | |||
===Characterization=== | ===Characterization=== | ||
Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues. | Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues. | ||
| − | + | ||
| + | The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it. | ||
[[File:T--Tokyo_Tech--test-electrop.gif|thumb|left|300px| '''Figure 1:''' '''Result of the qualitative experiment''' - description comes here. ]] | [[File:T--Tokyo_Tech--test-electrop.gif|thumb|left|300px| '''Figure 1:''' '''Result of the qualitative experiment''' - description comes here. ]] | ||
<br> | <br> | ||
| − | As shown in | + | As shown in Figure 1, the band of DENV1 C come around 300 bp. |
<br style="clear: both" /> | <br style="clear: both" /> | ||
===Analysis=== | ===Analysis=== | ||
| − | + | Our team successfully validated that the sequence of DENV1 C was synthesized, and obtained enough amount of it for the transduction. | |
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 14:02, 16 October 2018
DENV1 C (Capsid Protein | Dengue Virus Serotype I)
Usage and Biology
Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
Characterization
Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.
The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
As shown in Figure 1, the band of DENV1 C come around 300 bp.
Analysis
Our team successfully validated that the sequence of DENV1 C was synthesized, and obtained enough amount of it for the transduction.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
参考文献 - NCBI Gene Database - Byk LA, GamarnikAV. Properties and Functions of the Dengue Virus Capsid Protein. Annu Rev Virol. 2016 Sep 29;3(1):263-281. Epub 2016 Aug 3. Review.PubMed PMID: 27501261; PubMed Central PMCID: PMC5417333.