Difference between revisions of "Part:BBa K2587018:Design"

 
(→‎Source)
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The pRpa sequence as well as the lysis gene E sequence was obtained from GenBank and codon optimised for E.coli. The other parts are from the CIDAR MoClo toolbox.  
+
The p<sub><i>Rpa</i></sub> sequence as well as the lysis gene <i>E</i> sequence was obtained from GenBank and codon optimised for <i>E. coli</i>. The other parts are from the CIDAR MoClo toolbox.
  
 
===References===
 
===References===

Latest revision as of 13:51, 16 October 2018


Prpa_RBS_E_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93


Design Notes

The sequence is optimised for the use with the CIDAR MoClo Toolbox.


Source

The pRpa sequence as well as the lysis gene E sequence was obtained from GenBank and codon optimised for E. coli. The other parts are from the CIDAR MoClo toolbox.

References