Difference between revisions of "Part:BBa K2587016:Design"

 
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===Source===
 
===Source===
  
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The RpaR sequence was obtained from GenBank and codon optimised for E.coli. The remaining sequences were obtained from the CIDAR MoClo toolbox.
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The RpaR sequence was obtained from GenBank and codon optimised for <i>E. coli</i>. The remaining sequences were obtained from the CIDAR MoClo toolbox.
  
 
===References===
 
===References===

Latest revision as of 13:46, 16 October 2018


P_RBS_rpaR_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 67


Design Notes

The part is optimised for the use Golden Gate Cloning using the CIDAR MoClo toolbox.


Source

The RpaR sequence was obtained from GenBank and codon optimised for E. coli. The remaining sequences were obtained from the CIDAR MoClo toolbox.

References