Difference between revisions of "Part:BBa K2587005:Design"
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===Source=== | ===Source=== | ||
| − | The sequence is from GenBank and codon optimised for <i>E.coli</i>. | + | The sequence is from GenBank and codon optimised for <i>E. coli</i>. |
===References=== | ===References=== | ||
Latest revision as of 13:43, 16 October 2018
lasR
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 579
Illegal SpeI site found at 738 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 579
Illegal SpeI site found at 738 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 579
Illegal SpeI site found at 738 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 579
Illegal SpeI site found at 738 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 731
Design Notes
The sequence contains Type II S restriction sites and is optimised for the use in the CIDAR toolbox.
Source
The sequence is from GenBank and codon optimised for E. coli.