Difference between revisions of "Part:BBa K2587013:Design"

 
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===Source===
 
===Source===
  
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The sequence for LuxI is obtained from Genbank and codon optimised for E.coli. The other parts are obtained from the CIDAR MoClo toolkit.
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The sequence for LuxI is obtained from Genbank and codon optimised for <i>E. coli</i>. The other parts are obtained from the CIDAR MoClo toolkit.
  
 
===References===
 
===References===

Latest revision as of 13:42, 16 October 2018


P_RBS_luxI_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construct is optimised for the use with the CIDAR MoClo Toolbox. It contains Type II S restriction sites.


Source

The sequence for LuxI is obtained from Genbank and codon optimised for E. coli. The other parts are obtained from the CIDAR MoClo toolkit.

References