Difference between revisions of "Part:BBa K2610021"
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<partinfo>BBa_K2610021 short</partinfo> | <partinfo>BBa_K2610021 short</partinfo> | ||
| − | The gene rpoE encodes the sigma factor E, a protein in Escherichia coli and other species of bacteria. RpoE appears to be necessary for the exocytoplasmic stress response. E. coli mutants without rpoE are not able to grow at temperatures above 42 degrees Celsius. | + | The gene rpoE encodes the sigma factor E, a protein in Escherichia coli and other species of bacteria. RpoE appears to be necessary for the exocytoplasmic stress response. E. coli mutants without rpoE are not able to grow at temperatures above 42 degrees Celsius.<span class="plainlinks">[https://jb.asm.org/content/177/10/2918 (Hiratsu et al, 1995)]</span> |
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| + | For more information please refer to our <span class="plainlinks">[http://2018.igem.org/Team:Leiden/Results Results page]</span> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 13:34, 16 October 2018
pRpoE
The gene rpoE encodes the sigma factor E, a protein in Escherichia coli and other species of bacteria. RpoE appears to be necessary for the exocytoplasmic stress response. E. coli mutants without rpoE are not able to grow at temperatures above 42 degrees Celsius.(Hiratsu et al, 1995)
For more information please refer to our [http://2018.igem.org/Team:Leiden/Results Results page]
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the RpoE promoter is activated by, amongst others, chloramphenicol, ethanol and nalidixic acid.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]