Difference between revisions of "Part:BBa K2817006"
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| − | + | The mRNA mediated by the cold-acting promoter CspA can only be efficiently translated at a low temperature of, for example, 16 ℃, so we use it to activate the expression of mazF at low temperatures. In this way, when the engineered bacteria leaves the intestine and enters the environment, it will be killed by our cold shock kill switch. | |
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Revision as of 13:19, 16 October 2018
PcspA-RBS-mazF
The mRNA mediated by the cold-acting promoter CspA can only be efficiently translated at a low temperature of, for example, 16 ℃, so we use it to activate the expression of mazF at low temperatures. In this way, when the engineered bacteria leaves the intestine and enters the environment, it will be killed by our cold shock kill switch.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]