Difference between revisions of "Part:BBa K2644003"

 
 
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<partinfo>BBa_K2644003 short</partinfo>
 
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Arsenic binding repressor protein--ArsR and ArsR promoter.
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K2644003 parameters</partinfo>
 
<partinfo>BBa_K2644003 parameters</partinfo>
 
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===Usage and Biology===
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This part consists of the ArsR repressor binding site and the ArsR gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter.
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===Results===
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We used this part to build a new arsenic induction loop through it. Therefore, we first added 10μl ddH2O to the corresponding hole, and then took PCR was performed at 1 μl, and the corresponding DNA fragment was obtained.
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  https://static.igem.org/mediawiki/2018/1/11/T--TJU_China--1-8.png<br>
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Figure1. The result of nucleic acid gel electrophoresis of Bba-J33201 after PCR. Lane M, Marker. Lane 1-6，Bba-J33201
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<br>
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Then we performed PCR on the promoter fragment and ArsR fragment in this fragment, and the results are as follows.
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  https://static.igem.org/mediawiki/2018/8/88/T--TJU_China--1-9.png<br>
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Figure2. The result of nucleic acid gel electrophoresis of Ars Promoter after PCR. lane M, Marker. Lane 1-4, Ars Promoter.
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<br>
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  https://static.igem.org/mediawiki/2018/a/ad/T--TJU_China--1-10.png<br>
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Figure 3. The result of nucleic acid gel electrophoresis of ArsR Protein after PCR. Lane M, Marker. Line1, ArsR Protein.
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<br>

Latest revision as of 13:19, 16 October 2018


ArsR

Arsenic binding repressor protein--ArsR and ArsR promoter. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This part consists of the ArsR repressor binding site and the ArsR gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter.

Results

We used this part to build a new arsenic induction loop through it. Therefore, we first added 10μl ddH2O to the corresponding hole, and then took PCR was performed at 1 μl, and the corresponding DNA fragment was obtained. 

  T--TJU_China--1-8.png

Figure1. The result of nucleic acid gel electrophoresis of Bba-J33201 after PCR. Lane M, Marker. Lane 1-6，Bba-J33201

Then we performed PCR on the promoter fragment and ArsR fragment in this fragment, and the results are as follows. 

  T--TJU_China--1-9.png

Figure2. The result of nucleic acid gel electrophoresis of Ars Promoter after PCR. lane M, Marker. Lane 1-4, Ars Promoter. 

  T--TJU_China--1-10.png

Figure 3. The result of nucleic acid gel electrophoresis of ArsR Protein after PCR. Lane M, Marker. Line1, ArsR Protein.