Difference between revisions of "Part:BBa K2686005:Experience"

Revision as of 13:13, 16 October 2018

The part was designed and constructed in a pET14 vector. The sequence was confirmed using Sanger sequencing. Since this part is an intermediary only built for cloning purposes, the part was never expressed by itself in cell-free, but was ligated with inserts (OT1 coding sequences in our case) using Golden Gate assemblies with BsaI.

Applications of BBa_K2686005

User Reviews

UNIQ03322399d803678a-partinfo-00000000-QINU UNIQ03322399d803678a-partinfo-00000001-QINU

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-The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was expressed in the pET vector using a cell-free expression system (See results on EPFL_2018 team wiki).
-The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.
-__NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter how you used this part and how it worked out.The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing.
+The part was designed and constructed in a pET14 vector. The sequence was confirmed using Sanger sequencing.
+Since this part is an intermediary only built for cloning purposes, the part was never expressed by itself in cell-free, but was ligated with inserts (OT1 coding sequences in our case) using Golden Gate assemblies with BsaI.
 ===Applications of BBa_K2686005===