Difference between revisions of "Part:BBa K2582006"

 
 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2582006 short</partinfo>
 
<partinfo>BBa_K2582006 short</partinfo>
  
a
+
This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP)  [https://parts.igem.org/Part:BBa_K2582005 BBa_K2582005]. It targets the influenza Hemagglutinin subtype 3 (Gene accession number: NC_007366.1) by nucleotide 1305-1326.
  
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 
+
<p>This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of <i>E. coli</i>, it can be expressed by IPTG induction.</p>
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 12:58, 16 October 2018

T7 Promoter-RAP(H3-1305-1326)-T7 Terminator

This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP) BBa_K2582005. It targets the influenza Hemagglutinin subtype 3 (Gene accession number: NC_007366.1) by nucleotide 1305-1326.

Usage and Biology

This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of E. coli, it can be expressed by IPTG induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]