Difference between revisions of "Part:BBa K2587030"
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<partinfo>BBa_K2587030 short</partinfo> | <partinfo>BBa_K2587030 short</partinfo> | ||
| − | Transcription unit with the gene ddh coding for Meso-diaminopimelate D-dehydrogenase. The enzyme catalyses the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate in the lysine biosynthesis pathway of Corynebacterium glutamicum.We use it as a multi step enzyme for the lysine biosynthesis in Escherichia coli, since the wiltype normally needs five enzymes for the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate. | + | Transcription unit with the gene <i>ddh</i> coding for Meso-diaminopimelate D-dehydrogenase. The enzyme catalyses the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate in the lysine biosynthesis pathway of <i>Corynebacterium glutamicum</i><sup>1</sup>.We use it as a multi step enzyme for the lysine biosynthesis in <i>Escherichia coli</i>, since the wiltype normally needs five enzymes for the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate. |
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<partinfo>BBa_K2587030 parameters</partinfo> | <partinfo>BBa_K2587030 parameters</partinfo> | ||
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| + | <p>1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in Corynebacterium glutamicium." Journal of bacteriology 173.14 (1991): 4510-4516.:<br> | ||
| + | https://jb.asm.org/content/173/14/4510.short</p> | ||
Revision as of 12:49, 16 October 2018
Pj23106_RBS_ddh_T
Transcription unit with the gene ddh coding for Meso-diaminopimelate D-dehydrogenase. The enzyme catalyses the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate in the lysine biosynthesis pathway of Corynebacterium glutamicum1.We use it as a multi step enzyme for the lysine biosynthesis in Escherichia coli, since the wiltype normally needs five enzymes for the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 422
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 390
Illegal NgoMIV site found at 615
Illegal AgeI site found at 825 - 1000COMPATIBLE WITH RFC[1000]
1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in Corynebacterium glutamicium." Journal of bacteriology 173.14 (1991): 4510-4516.:
https://jb.asm.org/content/173/14/4510.short