Difference between revisions of "Part:BBa K2587028"
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<p><h4><strong>References</strong></h4></p>
 
<p><h4><strong>References</strong></h4></p>
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<p>1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in Corynebacterium glutamicium." Journal of bacteriology 173.14 (1991): 4510-4516.:<br>
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https://jb.asm.org/content/173/14/4510.short</p>

Revision as of 12:25, 16 October 2018


ddh

Gene coding for Meso-diaminopimelate D-dehydrogenase. The enzyme catalyses the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate in the lysine biosynthesis pathway of Corynebacterium glutamicum1. We use it as a multi step enzyme for the lysine biosynthesis in Escherichia coli, since the wiltype normally needs five enzymes for the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 358
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 551
    Illegal AgeI site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in Corynebacterium glutamicium." Journal of bacteriology 173.14 (1991): 4510-4516.:
https://jb.asm.org/content/173/14/4510.short