Difference between revisions of "Part:BBa K2560011:Design"

Revision as of 10:57, 16 October 2018

Phytobrick version of 5'Connector Dummy

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 1
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
Illegal NotI site found at 7
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 1
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The sequence was synthesized in silico by Daniel Stukenberg and ordered from Integrated DNA Technologies(IDT).

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGAACAGAATTCGCGGCCGCTTCTAGAGGGAG Reverse Oligo: CTCACTCCCTCTAGAAGCGGCCGCGAATTCTGTT

@@ Line 4: / Line 4: @@
 <partinfo>BBa_K2560011 SequenceAndFeatures</partinfo>
 ===Design Notes===
-More information coming soon!
+<html>
+The sequence was synthesized <i> in silico </i> by Daniel Stukenberg and ordered from <a href="https://eu.idtdna.com/pages">Integrated DNA Technologies</a>(IDT).
+</html>
+===Source===
+<html>
+The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this
+<a href="https://parts.igem.org/Help:Promoters/Construction">site</a>.
+</html>
+<b> Forward Oligo:</b>
+CTCGAACAGAATTCGCGGCCGCTTCTAGAGGGAG
+<b> Reverse Oligo:</b>
+CTCACTCCCTCTAGAAGCGGCCGCGAATTCTGTT
-===Source===
-More information coming soon!
 ===References===