Difference between revisions of "Part:BBa K2812004:Design"

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===Source===
 
===Source===
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The initial starting sequence for truncated lysostaphin was taken from <partinfo>BBa_K748002</partinfo>. The amino acid sequence for HlyA was taken from uniprot (UniProtKB - Q1R2T5 (Q1R2T5_ECOUT)), residue 807-1024.<sup>1</sup> This is the C-terminal signal sequence, the N-terminal catalytic domain responsible for lysis of red blood cells is not included. The thrombin substrate sequence was obtained from Waugh, 2011<sup>2</sup> and all three components were linked via two flexible GGGGS linkers in between. This amino acid sequence was used to generate a codon optimized sequence for expression in ''E. coli''.
 
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The designed sequence was synthesized ''de novo'' by IDT.
 
The designed sequence was synthesized ''de novo'' by IDT.
  
 
===References===
 
===References===
 
1) https://www.uniprot.org/uniprot/Q1R2T5#sequences
 
1) https://www.uniprot.org/uniprot/Q1R2T5#sequences

Revision as of 08:45, 16 October 2018


Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1351
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Total DNA sequence has been codon optimized for E. coli.


Source

The designed sequence was synthesized de novo by IDT.

References

1) https://www.uniprot.org/uniprot/Q1R2T5#sequences