Difference between revisions of "Part:BBa K2764003"

Revision as of 08:36, 16 October 2018

Alkane channel protein with BBa_J23110 Constitutive promoter

Usage and biology

As shown in literature, the AlkL protein may be toxic and inhibit the growth of its host. We decided to test the severity of this toxicity and compare by using Anderson promoters of varying strength. BBa_K2764001 utilizes the promoter BBa_J23104, which gives the biobrick a strong promoter strength of 0.7 on the Anderson promoter scale. This will be used to measure the effect of toxicity of AlkL based on the growth curve of bacteria transformed with this biobrick. In this experiment, we used a bacteria transformed with a plasmid containing insert of BBa_I13401 as the control. This is because the control's plasmid has a similar size to our part's plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 712
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 364
Illegal NgoMIV site found at 577
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2764003 short</partinfo>
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+<b>Usage and biology</b> <br>
+<p> As shown in literature, the AlkL protein may be toxic and inhibit the growth of its host. We decided to test the severity of this toxicity and compare by using Anderson promoters of varying strength. BBa_K2764001 utilizes the promoter BBa_J23104, which gives the biobrick a strong promoter strength of 0.7 on the Anderson promoter scale. This will be used to measure the effect of toxicity of AlkL based on the growth curve of bacteria transformed with this biobrick. In this experiment, we used a bacteria transformed with a plasmid containing insert of BBa_I13401 as the control. This is because the control's plasmid has a similar size to our part's plasmid. </p>
 <!-- Add more about the biology of this part here